Utilizing redox-sensitive GFP fusions to detect in vivo redox changes in a genetically engineered prokaryote.
Identifieur interne : 000101 ( Main/Exploration ); précédent : 000100; suivant : 000102Utilizing redox-sensitive GFP fusions to detect in vivo redox changes in a genetically engineered prokaryote.
Auteurs : Wilhad Hans Reuter [États-Unis] ; Thorsten Masuch [Allemagne] ; Na Ke [États-Unis] ; Marine Lenon [États-Unis] ; Meytal Radzinski [Israël] ; Vu Van Loi [Allemagne] ; Guoping Ren [États-Unis] ; Paul Riggs [États-Unis] ; Haike Antelmann [Allemagne] ; Dana Reichmann [Israël] ; Lars I. Leichert [Allemagne] ; Mehmet Berkmen [États-Unis]Source :
- Redox biology [ 2213-2317 ] ; 2019.
Descripteurs français
- KwdFr :
- Cellules procaryotes (métabolisme), Génie génétique (MeSH), Imagerie moléculaire (MeSH), Oxydoréduction (MeSH), Peroxyde d'hydrogène (métabolisme), Protéines de fusion recombinantes (génétique), Protéines de fusion recombinantes (métabolisme), Protéines à fluorescence verte (génétique), Protéines à fluorescence verte (métabolisme), Stress oxydatif (MeSH), Techniques de biocapteur (MeSH).
- MESH :
- génétique : Protéines de fusion recombinantes, Protéines à fluorescence verte.
- métabolisme : Cellules procaryotes, Peroxyde d'hydrogène, Protéines de fusion recombinantes, Protéines à fluorescence verte.
- Génie génétique, Imagerie moléculaire, Oxydoréduction, Stress oxydatif, Techniques de biocapteur.
English descriptors
- KwdEn :
- Biosensing Techniques (MeSH), Genetic Engineering (MeSH), Green Fluorescent Proteins (genetics), Green Fluorescent Proteins (metabolism), Hydrogen Peroxide (metabolism), Molecular Imaging (MeSH), Oxidation-Reduction (MeSH), Oxidative Stress (MeSH), Prokaryotic Cells (metabolism), Recombinant Fusion Proteins (genetics), Recombinant Fusion Proteins (metabolism).
- MESH :
- chemical , genetics : Green Fluorescent Proteins, Recombinant Fusion Proteins.
- chemical , metabolism : Green Fluorescent Proteins, Hydrogen Peroxide, Recombinant Fusion Proteins.
- metabolism : Prokaryotic Cells.
- Biosensing Techniques, Genetic Engineering, Molecular Imaging, Oxidation-Reduction, Oxidative Stress.
Abstract
Understanding the in vivo redox biology of cells is a complex albeit important biological problem. Studying redox processes within living cells without physical disruption or chemical modifications is essential in determining the native redox states of cells. In this study, the previously characterized reduction-oxidation sensitive green fluorescent protein (roGFP2) was used to elucidate the redox changes of the genetically engineered Escherichia coli strain, SHuffle. SHuffle cells were demonstrated to be under constitutive oxidative stress and responding transcriptionally in an OxyR-dependent manner. Using roGFP2 fused to either glutathione (GSH)- or hydrogen peroxide (H2O2)- sensitive proteins (glutaredoxin 1 or Orp1), the cytosolic redox state of both wild type and SHuffle cells based on GSH/GSSG and H2O2 pools was measured. These probes open the path to in vivo studies of redox changes and genetic selections in prokaryotic hosts.
DOI: 10.1016/j.redox.2019.101280
PubMed: 31450103
PubMed Central: PMC6831853
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Ruhr University Bochum, Institute of Biochemistry and Pathobiochemistry, Microbial Biochemistry, Universitätsstr. 150, 44780, Bochum, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>New England Biolabs, 240 County Road, Ipswich, MA, 01938, USA; Ruhr University Bochum, Institute of Biochemistry and Pathobiochemistry, Microbial Biochemistry, Universitätsstr. 150, 44780, Bochum</wicri:regionArea><wicri:noRegion>44780, Bochum</wicri:noRegion><wicri:noRegion>44780, Bochum</wicri:noRegion><wicri:noRegion>Bochum</wicri:noRegion></affiliation></author><author><name sortKey="Ke, Na" sort="Ke, Na" uniqKey="Ke N" first="Na" last="Ke">Na Ke</name><affiliation wicri:level="1"><nlm:affiliation>New England Biolabs, 240 County Road, Ipswich, MA, 01938, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>New England Biolabs, 240 County Road, Ipswich, MA, 01938</wicri:regionArea><wicri:noRegion>01938</wicri:noRegion></affiliation></author><author><name sortKey="Lenon, Marine" sort="Lenon, Marine" uniqKey="Lenon M" first="Marine" last="Lenon">Marine Lenon</name><affiliation wicri:level="1"><nlm:affiliation>New England Biolabs, 240 County Road, Ipswich, MA, 01938, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>New England Biolabs, 240 County Road, Ipswich, MA, 01938</wicri:regionArea><wicri:noRegion>01938</wicri:noRegion></affiliation></author><author><name sortKey="Radzinski, Meytal" sort="Radzinski, Meytal" uniqKey="Radzinski M" first="Meytal" last="Radzinski">Meytal Radzinski</name><affiliation wicri:level="1"><nlm:affiliation>The Hebrew University of Jerusalem, The Alexander Silberman Institute of Life Sciences, Safra Campus Givat Ram, Jerusalem, 91904, Israel.</nlm:affiliation><country xml:lang="fr">Israël</country><wicri:regionArea>The Hebrew University of Jerusalem, The Alexander Silberman Institute of Life Sciences, Safra Campus Givat Ram, Jerusalem, 91904</wicri:regionArea><wicri:noRegion>91904</wicri:noRegion></affiliation></author><author><name sortKey="Van Loi, Vu" sort="Van Loi, Vu" uniqKey="Van Loi V" first="Vu" last="Van Loi">Vu Van Loi</name><affiliation wicri:level="3"><nlm:affiliation>Institute for Biology-Microbiology, Freie Universität Berlin, 14195, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Biology-Microbiology, Freie Universität Berlin, 14195, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Ren, Guoping" sort="Ren, Guoping" uniqKey="Ren G" first="Guoping" last="Ren">Guoping Ren</name><affiliation wicri:level="1"><nlm:affiliation>New England Biolabs, 240 County Road, Ipswich, MA, 01938, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>New England Biolabs, 240 County Road, Ipswich, MA, 01938</wicri:regionArea><wicri:noRegion>01938</wicri:noRegion></affiliation></author><author><name sortKey="Riggs, Paul" sort="Riggs, Paul" uniqKey="Riggs P" first="Paul" last="Riggs">Paul Riggs</name><affiliation wicri:level="1"><nlm:affiliation>New England Biolabs, 240 County Road, Ipswich, MA, 01938, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>New England Biolabs, 240 County Road, Ipswich, MA, 01938</wicri:regionArea><wicri:noRegion>01938</wicri:noRegion></affiliation></author><author><name sortKey="Antelmann, Haike" sort="Antelmann, Haike" uniqKey="Antelmann H" first="Haike" last="Antelmann">Haike Antelmann</name><affiliation wicri:level="3"><nlm:affiliation>Institute for Biology-Microbiology, Freie Universität Berlin, 14195, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute for Biology-Microbiology, Freie Universität Berlin, 14195, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Reichmann, Dana" sort="Reichmann, Dana" uniqKey="Reichmann D" first="Dana" last="Reichmann">Dana Reichmann</name><affiliation wicri:level="1"><nlm:affiliation>The Hebrew University of Jerusalem, The Alexander Silberman Institute of Life Sciences, Safra Campus Givat Ram, Jerusalem, 91904, Israel.</nlm:affiliation><country xml:lang="fr">Israël</country><wicri:regionArea>The Hebrew University of Jerusalem, The Alexander Silberman Institute of Life Sciences, Safra Campus Givat Ram, Jerusalem, 91904</wicri:regionArea><wicri:noRegion>91904</wicri:noRegion></affiliation></author><author><name sortKey="Leichert, Lars I" sort="Leichert, Lars I" uniqKey="Leichert L" first="Lars I" last="Leichert">Lars I. Leichert</name><affiliation wicri:level="1"><nlm:affiliation>Ruhr University Bochum, Institute of Biochemistry and Pathobiochemistry, Microbial Biochemistry, Universitätsstr. 150, 44780, Bochum, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Ruhr University Bochum, Institute of Biochemistry and Pathobiochemistry, Microbial Biochemistry, Universitätsstr. 150, 44780, Bochum</wicri:regionArea><wicri:noRegion>44780, Bochum</wicri:noRegion><wicri:noRegion>44780, Bochum</wicri:noRegion><wicri:noRegion>Bochum</wicri:noRegion></affiliation></author><author><name sortKey="Berkmen, Mehmet" sort="Berkmen, Mehmet" uniqKey="Berkmen M" first="Mehmet" last="Berkmen">Mehmet Berkmen</name><affiliation wicri:level="1"><nlm:affiliation>New England Biolabs, 240 County Road, Ipswich, MA, 01938, USA. Electronic address: berkmen@neb.com.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>New England Biolabs, 240 County Road, Ipswich, MA, 01938</wicri:regionArea><wicri:noRegion>01938</wicri:noRegion></affiliation></author></analytic><series><title level="j">Redox biology</title><idno type="eISSN">2213-2317</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biosensing Techniques (MeSH)</term><term>Genetic Engineering (MeSH)</term><term>Green Fluorescent Proteins (genetics)</term><term>Green Fluorescent Proteins (metabolism)</term><term>Hydrogen Peroxide (metabolism)</term><term>Molecular Imaging (MeSH)</term><term>Oxidation-Reduction (MeSH)</term><term>Oxidative Stress (MeSH)</term><term>Prokaryotic Cells (metabolism)</term><term>Recombinant Fusion Proteins (genetics)</term><term>Recombinant Fusion Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cellules procaryotes (métabolisme)</term><term>Génie génétique (MeSH)</term><term>Imagerie moléculaire (MeSH)</term><term>Oxydoréduction (MeSH)</term><term>Peroxyde d'hydrogène (métabolisme)</term><term>Protéines de fusion recombinantes (génétique)</term><term>Protéines de fusion recombinantes (métabolisme)</term><term>Protéines à fluorescence verte (génétique)</term><term>Protéines à fluorescence verte (métabolisme)</term><term>Stress oxydatif (MeSH)</term><term>Techniques de biocapteur (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Green Fluorescent Proteins</term><term>Recombinant Fusion Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Green Fluorescent Proteins</term><term>Hydrogen Peroxide</term><term>Recombinant Fusion Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines de fusion recombinantes</term><term>Protéines à fluorescence verte</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Prokaryotic Cells</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellules procaryotes</term><term>Peroxyde d'hydrogène</term><term>Protéines de fusion recombinantes</term><term>Protéines à fluorescence verte</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biosensing Techniques</term><term>Genetic Engineering</term><term>Molecular Imaging</term><term>Oxidation-Reduction</term><term>Oxidative Stress</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Génie génétique</term><term>Imagerie moléculaire</term><term>Oxydoréduction</term><term>Stress oxydatif</term><term>Techniques de biocapteur</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Understanding the in vivo redox biology of cells is a complex albeit important biological problem. Studying redox processes within living cells without physical disruption or chemical modifications is essential in determining the native redox states of cells. In this study, the previously characterized reduction-oxidation sensitive green fluorescent protein (roGFP2) was used to elucidate the redox changes of the genetically engineered Escherichia coli strain, SHuffle. SHuffle cells were demonstrated to be under constitutive oxidative stress and responding transcriptionally in an OxyR-dependent manner. Using roGFP2 fused to either glutathione (GSH)- or hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)- sensitive proteins (glutaredoxin 1 or Orp1), the cytosolic redox state of both wild type and SHuffle cells based on GSH/GSSG and H<sub>2</sub>O<sub>2</sub> pools was measured. These probes open the path to in vivo studies of redox changes and genetic selections in prokaryotic hosts.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31450103</PMID><DateCompleted><Year>2020</Year><Month>02</Month><Day>27</Day></DateCompleted><DateRevised><Year>2020</Year><Month>02</Month><Day>27</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">2213-2317</ISSN><JournalIssue CitedMedium="Internet"><Volume>26</Volume><PubDate><Year>2019</Year><Month>09</Month></PubDate></JournalIssue><Title>Redox biology</Title><ISOAbbreviation>Redox Biol</ISOAbbreviation></Journal><ArticleTitle>Utilizing redox-sensitive GFP fusions to detect in vivo redox changes in a genetically engineered prokaryote.</ArticleTitle><Pagination><MedlinePgn>101280</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S2213-2317(19)30641-X</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.redox.2019.101280</ELocationID><Abstract><AbstractText>Understanding the in vivo redox biology of cells is a complex albeit important biological problem. Studying redox processes within living cells without physical disruption or chemical modifications is essential in determining the native redox states of cells. In this study, the previously characterized reduction-oxidation sensitive green fluorescent protein (roGFP2) was used to elucidate the redox changes of the genetically engineered Escherichia coli strain, SHuffle. SHuffle cells were demonstrated to be under constitutive oxidative stress and responding transcriptionally in an OxyR-dependent manner. Using roGFP2 fused to either glutathione (GSH)- or hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)- sensitive proteins (glutaredoxin 1 or Orp1), the cytosolic redox state of both wild type and SHuffle cells based on GSH/GSSG and H<sub>2</sub>O<sub>2</sub> pools was measured. These probes open the path to in vivo studies of redox changes and genetic selections in prokaryotic hosts.</AbstractText><CopyrightInformation>Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Reuter</LastName><ForeName>Wilhad Hans</ForeName><Initials>WH</Initials><AffiliationInfo><Affiliation>New England Biolabs, 240 County Road, Ipswich, MA, 01938, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Masuch</LastName><ForeName>Thorsten</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>New England Biolabs, 240 County Road, Ipswich, MA, 01938, USA; Ruhr University Bochum, Institute of Biochemistry and Pathobiochemistry, Microbial Biochemistry, Universitätsstr. 150, 44780, Bochum, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ke</LastName><ForeName>Na</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>New England Biolabs, 240 County Road, Ipswich, MA, 01938, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lenon</LastName><ForeName>Marine</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>New England Biolabs, 240 County Road, Ipswich, MA, 01938, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Radzinski</LastName><ForeName>Meytal</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>The Hebrew University of Jerusalem, The Alexander Silberman Institute of Life Sciences, Safra Campus Givat Ram, Jerusalem, 91904, Israel.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Van Loi</LastName><ForeName>Vu</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Institute for Biology-Microbiology, Freie Universität Berlin, 14195, Berlin, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ren</LastName><ForeName>Guoping</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>New England Biolabs, 240 County Road, Ipswich, MA, 01938, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Riggs</LastName><ForeName>Paul</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>New England Biolabs, 240 County Road, Ipswich, MA, 01938, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Antelmann</LastName><ForeName>Haike</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Institute for Biology-Microbiology, Freie Universität Berlin, 14195, Berlin, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Reichmann</LastName><ForeName>Dana</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>The Hebrew University of Jerusalem, The Alexander Silberman Institute of Life Sciences, Safra Campus Givat Ram, Jerusalem, 91904, Israel.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Leichert</LastName><ForeName>Lars I</ForeName><Initials>LI</Initials><AffiliationInfo><Affiliation>Ruhr University Bochum, Institute of Biochemistry and Pathobiochemistry, Microbial Biochemistry, Universitätsstr. 150, 44780, Bochum, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Berkmen</LastName><ForeName>Mehmet</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>New England Biolabs, 240 County Road, Ipswich, MA, 01938, USA. Electronic address: berkmen@neb.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>07</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Redox Biol</MedlineTA><NlmUniqueID>101605639</NlmUniqueID><ISSNLinking>2213-2317</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011993">Recombinant Fusion Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>147336-22-9</RegistryNumber><NameOfSubstance UI="D049452">Green Fluorescent Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>BBX060AN9V</RegistryNumber><NameOfSubstance UI="D006861">Hydrogen Peroxide</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D015374" MajorTopicYN="N">Biosensing Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005818" MajorTopicYN="N">Genetic Engineering</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D049452" MajorTopicYN="N">Green Fluorescent Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006861" MajorTopicYN="N">Hydrogen Peroxide</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D057054" MajorTopicYN="N">Molecular Imaging</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010084" MajorTopicYN="Y">Oxidation-Reduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018384" MajorTopicYN="N">Oxidative Stress</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011387" MajorTopicYN="N">Prokaryotic Cells</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011993" MajorTopicYN="N">Recombinant Fusion Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Disulfide bond formation</Keyword><Keyword MajorTopicYN="Y">Redox</Keyword><Keyword MajorTopicYN="Y">SHuffle</Keyword><Keyword MajorTopicYN="Y">roGFP</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>06</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>07</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>07</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>8</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>2</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>8</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31450103</ArticleId><ArticleId IdType="pii">S2213-2317(19)30641-X</ArticleId><ArticleId IdType="doi">10.1016/j.redox.2019.101280</ArticleId><ArticleId 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